Part:BBa_K1639008:Design

Tev Protease, single S219V mutation in the internal cleavage site

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2
  Illegal XhoI site found at 744
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 605
  

Design Notes

This part contains an additional BamHI restriction site at beginning of Tev Protease sequence and a XhoI restriction site at the end.

Source

Tobacco etch virus (TEV).